Comparison PCR and Culture methods for detect Coccoid forms of Helicobacter pylori

Abstract

Background:

Helicobacter pylori (Pylori) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. Environmental factors such as water a reservoir of H.pylori which infect human.  a Non-culture bacteria in coccoid forms widespread in aquatic environments.The objective of this study  was elevating the diagnostic value of PCR and culture methods for for diagnosis coccoid forms of H.pylori.

Materials and Methods:

To induce coccoid forms, ten different strains of H.pylori (H₁-H₁₀) were inoculated into 30 drinking water samples. Then, the samples were incubated at three different temperatures of 4°C, 22°C and 37°C for the durations of 30 and 60 days. The samples were cultured on brucella blood agar and DNA was also extracted also from them and PCR performed on samples

Results:

percentage of H.pylori cells detected at specified temperatures by the culture were 0%, 10% and 0% in the first month and were 0%,10%,30% in the second month whereas by the PCR molecular method were 30%, 80%, and 30% in the first month and were 20%, 20%, and 40% in the second month, respectively.

Conclusion:

finding show PCR  methodis more capable than culture  fordetect the coccoid forms of H.pylori, therefore this method could be used to detect non- culture forms.