Assessment of Matrix Metalloproteinase-14 Gene Polymorphisms (+7096) in Egyptian Patients with Hepatocellular Carcinoma

Abstract

Background:

Hepatocellular carcinoma (HCC) ranks as the first and the second malignancy in men and women in Egypt. Many inflammatory markers are included in HCC pathogenesis. Matrix metalloproteinase-14 (MMP-14) is one of them and it is implicated in regulating HCC tumor progression and prognosis. This study was done basically to assess the frequency of alleles of rs2236307 single nucleotide polymorphism (SNPs) of the MMP-14 (+7096) gene in patients with HCC and to determine if there is an association between its alleles polymorphism and the occurrence of HCC in Egyptian patients.

Materials and Methods:

A case-control study was done in the Tropical Medicine Department at Zagazig University Hospitals and the Medical Biochemistry Department, Faculty of Medicine, Zagazig University. The study included 540 subjects, classified into three groups HCC, cirrhotic, and controls (180 in each group). MMP-14 gene polymorphism analysis in the promoter of the MMP-14 gene rs2236307 was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Results:

The TC genotype was significantly higher among patients with HCC when compared with the control group (77.8% versus 16.7%, respectively). The TC genotype was higher among patients with HCC compared with the cirrhotic group (77.8% versus 38.9%, respectively). Metastatic lesions and portal vein thrombosis were significantly higher among the TC group compared with the CC and the TT groups in the HCC group.

Conclusion:

The polymorphisms of MMP-14 rs2236307 increase the risk of HCC. Both the TC and the CC genotypes showed HCC risk association but the the CC genotype appeared with lower potential.