Pre- and Post-Treatment Effects of Lactobacillus acidophilus ATCC 4356 on Oxidative Stress and Function of Liver in Diabetic Male Rats

Abstract

Background:

This study investigated the effects of lactobacillus acidophilus ATCC4356 on the oxidant and antioxidant factors of the liver and levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) before and after streptozotocin-induced diabetes in male rats.

Materials and Methods:

Thirty male Wistar rats were divided into five groups (n=6): Control (C), Control probiotic (CP), Diabetic (D), Diabetic Pretreatment with lactobacillus (DPB[A1] ), and Post-treatment with lactobacillus (DPA) groups. C group received daily 1 mL of normal saline for 6 weeks. CP group received daily 1×10⁹ cfu/mL L. acidophilus ATCC 4356 for 6 weeks. D group received daily 1mL normal saline for 2 weeks before and for 4 weeks after diabetes induction. DPB group received daily 1×10⁹ cfu/ml L. acidophilus ATCC 4356 for 2 weeks before and for 4 weeks after diabetes induction. DPA group first received daily 1mL normal saline for 2 weeks before diabetes and then received daily 1×10⁹ cfu/mL L. acidophilus ATCC 4356 for 4 weeks after it.

Results:

 L. acidophilus ATCC 4356 decreased liver malondialdehyde (MDA) and H₂O₂ concentration and serum AST significantly in both pre- and post-treatment groups compared with the D group. Catalase activity (CAT) and serum ALT showed a significant decrease in the post-treatment group compared with the D group. Glutathione peroxidase (GPx) activity showed a significant increase in the post-treatment group compared to the D group.

Conclusion:

The present study showed that L. acidophilus ATCC4356 had more protective effects on the liver in the post-treatment group compared with the pretreatment one.