The Effect of Harmaline on Aspartate Aminotransferase, Alanine Aminotransferase, and Albumin in Normal and Diabetic Rats

Abstract

Background:

Currently, diabetes is one of the most common diseases that has a devastating effect on the body and causes free radicals in the body. One of the most important organs in the body that metabolizes and analyzes hormones and various substances is the liver (the largest gland of the body. Therefore, in order to cope with the damaging effects of diabetes and its effect on some liver enzymes, strong antioxidants have been needed. In this study, we tried to assess the effects of harmaline on albumin and some liver enzymes.

Materials and Methods:

In this study, 35 male vistar rats with a weight range of 180-200 g were divided into five groups of seven in control group (that received citrate buffer as they became diabetic) and case group (diabetic with intraperitoneal injection of 55 mg/kg streptozotocin),  experimental group 1 (diabetic and subcutaneously receiving 10 mg/kg harmaline), experimental group 2 (diabetic and received subcutaneous dose of 15 mg/kg of harmaline), and experimental group 3 (diabetic and received 20 mg/kg of harmaline subcutaneously) and After two months of becoming diabetic, rats received subcutaneous medication of various doses of harmaline for two weeks. Then, anesthesia was achieved in rats with ketamine and zylazine, and blood sampling was conducted directly from the heart. After separating serum to measure glucose and insulin, samples were delivered to the laboratory.

Results:

In this study, a significant decrease was observed in the amount of albumin in the experimental groups compared with the control group and a significant increase was observed in the amount of aspartate aminotransferase, and alanine aminotransferase enzymes in experimental groups compared with the control group. In experimental diabetic groups, the amount of albumin has also shown no significant changes compared with the other diabetic groups.

Conclusion:

 This study showed that taking harmaline caused a disturbance in the balance of liver enzymes and elevated serum albumin levels in healthy and diabetic rats.has changed the balance of liver enzymes and increased blood albumin in healthy rats, while liver enzymes increased in diabetic groups as dosage increased.